Gene tools

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Gene webpage

The gene webpage can be accessed either from the home webpage of the Listeriomics website or by double-clicking on a genome in the genomic webpage.

File:GenePageTuto.png
Printscreen of the Gene webpage of the Listeriomics website


General organization

Chromosome selection

Here one can select a specific genome among the 50 Listeria strains available. Then one has to select a chromosome. In Listeria species, in general the first one is a circular chromosome and the second one is a plasmid. With a star are indicated genomes for which transcriptomic datasets are available.

Gene search and selection

In this list, all the genes available for a specific chromosome are displayed. One can search for a gene locus, a gene name, or even key words which can be found in the annotation of each gene. A click on an element of the list will display all related information on the Gene information panel.

Omics panel selection

For each Listeria gene four different information panels are available.

  • General information: The position of the gene, possible annotated function, nucleotide sequence and amino-acid sequence.
  • Gene conservation in other Listeria strains : It dynamically displays on the Listeria phylogram presence of absence of homolog to the selected gene in each existing Listeria strain. It also displays a summary table of all homologs with their similarity percentages and amino acid sequences. One can then create a multi-alignment file of the homologs.
  • Expression atlas: It shows in which transcriptomics datasets the selected gene is differently expressed. The cut-off on the Log(Fold Change) value for detecting such elements can be changed by the user.
  • Protein atlas: It displays every proteomics dataset in which the protein coded by the selected gene has been detected.
Gene information panel

The panel in which all information will be displayed

How-to access gene annotation ?

File:GenepanelGeneralInfo.png
Gene annotation panel of the Gene webpage
Click on General information on the Omics panel selection
  • Genome viewer: Displays the gene in the genome viewer to see surrounding genome features.
  • General information: Every general information available on gene position, product, Cluster of Orthologous Gene (COG) and summary of gene conservation.
  • Supplementary information: All supplementary information available on the gene annotation such as conserved motif or alternative annotation.
  • Sequences: DNA and amino acid sequences are available here for copy/paste.

Is my gene conserved ?

Gene conservation panel of the Gene webpage
Click on General information on the Omics panel selection
  • Genome viewer: Displays the gene in the genome viewer to see surrounding genome features.
  • General information: Every general information available on gene position, product, Cluster of Orthologous Gene (COG) and summary of gene conservation.
  • Supplementary information: All supplementary information available on the gene annotation such as conserved motif or alternative annotation.
  • Sequences: DNA and amino acid sequences are available here for copy/paste.

Is my gene transcribed ?

Expression atlas panel of the Gene webpage
Click on General information on the Omics panel selection
  • Genome viewer: Displays the gene in the genome viewer to see surrounding genome features.
  • General information: Every general information available on gene position, product, Cluster of Orthologous Gene (COG) and summary of gene conservation.
  • Supplementary information: All supplementary information available on the gene annotation such as conserved motif or alternative annotation.
  • Sequences: DNA and amino acid sequences are available here for copy/paste.

Is my gene translated ?

Protein atlas panel of the Gene webpage
Click on General information on the Omics panel selection
  • Genome viewer: Displays the gene in the genome viewer to see surrounding genome features.
  • General information: Every general information available on gene position, product, Cluster of Orthologous Gene (COG) and summary of gene conservation.
  • Supplementary information: All supplementary information available on the gene annotation such as conserved motif or alternative annotation.
  • Sequences: DNA and amino acid sequences are available here for copy/paste.